Taq DNA Polymerase, recombinant (5 U/µL)

Highlights

• Thermostable—half life is more than 40 min at 95°C
• Generates PCR products with 3′-dA overhangs
• Supplied with two buffers—10X Taq Buffer with KCl and 10X Taq Buffer with (NH₄)₂SO₄. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming
• Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)

Applications

• Routine PCR amplification of DNA fragments up to 5 kb
• High throughput PCR
• DNA labeling

Note

• The error rate of Taq DNA Polymerase in PCR is 2.2 x 10^-5 errors per nt per cycle. Accordingly, the accuracy of PCR is 4.5 x 10⁴. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
• The 10X Taq Buffer without Detergent is recommended for microarray experiments.