Description
PROPERTIES
grade for molecular biology
Quality Level 200
assay ≥99.5%
form powder
technique(s) electrophoresis: suitable
impurities
0.05% Free carboxylic acid (T)
≤50 mg/kg Total sulfur (as SO4 ) (ICP)
conductivity ≤10 μmho (2% solution)
anion traces chloride (Cl–): ≤50 mg/kg
cation traces
Ca: ≤10 ppm
Cd: ≤5 ppm
Co: ≤5 ppm
Cr: ≤5 ppm
Cu: ≤5 ppm
Fe: ≤5 ppm
K: ≤50 ppm
Mg: ≤5 ppm
Mn: ≤5 ppm
Na: ≤50 ppm
Ni: ≤5 ppm
Pb: ≤5 ppm
Zn: ≤5 ppm
absorption ≤0.2 at 290 at 1%
foreign activity DNase and RNase, none detected
SMILES string C=CC(=O)NCNC(=O)C=C
InChI 1S/C7H10N2O2/c1-3-6(10)8-5-9-7(11)4-2/h3-4H,1-2,5H2,(H,8,10)(H,9,11)
InChI key ZIUHHBKFKCYYJD-UHFFFAOYSA-N
DESCRIPTION
General description
N,N′-Methylenebisacrylamide is commonly called as bis. It is used as a cross-linking agent in the preparation of polyacrylamide gels consisting of chains of acrylamide monomers. The concentration of N,N′-methylenebisacrylamide and acrylamide determines the pore size of the gel. The higher concentration of bis forms rigid gels with smaller pores due to more cross-linking leading to polymerization and vice versa.
Application
N,N′-Methylenebisacrylamide has been used:
- as a crosslinker in the monomer solution to prepare N,N-dimethyl acrylamide (DMAAm)-based cryogel and acrylamide (AAm)-based cryogel matrix
- as a crosslinker to prepare tough hydrogel network
- in the preparation of acrylamide-bisacrylamide stock solution for the preparation of polyacrylamide gel
To be mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis.
Packaging
25, 100, 250 g in poly bottle
SAFETY INFORMATION
Pictograms
GHS06,GHS08
Signal Word
Danger
Hazard Statements
H301 – H340
Precautionary Statements
P202 – P264 – P270 – P280 – P301 + P310 – P405
Hazard Classifications
Acute Tox. 3 Oral – Muta. 1B
Storage Class Code
6.1C – Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 2
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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