Murashige and Skoog Basal Medium

Murashige and Skoog Basal Medium has been used:

• to germinate the seeds for seedling growth and germination studies
• in the suspension culture of Nicotiana plumbaginifolia for transformation
• in the root culture of hairy clone A. artemisiifolia T4

1, 10 L

With the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins as described by Gamborg, et al. (1968).

Media Formulation

Formulated to contain 4.4 grams of powder per liter of medium.

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient, micronutrient and vitamin mixtures.
Murashige and Skoog medium (M0404) contains the macro- and micronutrients of M&S basal medium as described by Gamborg, et al (1968). It can be supplemented with organics and with auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.