suitable for microbiology, 
Synonym(s):
Luria Bertani Agar, high salt

CAT Number: BC-52062

Properties

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
casein enzymic hydrolysate, 10 g/L
sodium chloride, 10 g/L
yeast extract, 5 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.5±0.2 (25 °C)

application(s)

clinical testing
food and beverages
life science and biopharma

microbiology

suitability

nonselective for Escherichia coli
nonselective for bacteria (General Media)
nonselective for coliforms

Category:

Description

Description

General description

LB-Agar, high salt is a growth media used in microbiology. It supports Escherichia coli growth to an optical density at 600 nm (OD600) of 7.

Application

LB-Agar, high salt may be used as a growth media in the determination of phylogenetic groups (A, B1, B2, and D) of Escherichia coli bacteria using triplex PCR technique.
Luria Media are used for the cultivation and maintenance of recombinant strains of E. coli for genetic and molecular studies.

Preparation Note

Suspend 40 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Sterilize at 121°C for 15 minutes. Dispense as desired.
Safety Information

Storage Class Code

11 – Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves

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