Description
CAS Number:
299-11-6
Phenazine methosulfate
Storage Temperature -0 °C
Product Description
Molecular Formula: C14H14N2O4S
Molecular Weight: 306.3
CAS Number: 299-11-6
Extinction coefficient: EM for oxidized phenazine
methosulfate (PMS) at maximum wavelength 387 nm
is 26,300 (pH 2-8). The difference in molar extinction
coefficients between oxidized and reduced PMS is
25,000.1
Melting Point: 158-160 °C
PMS is an electron acceptor and carrier in enzyme
systems. The oxidized form is yellow and the reduced
form is colorless. Since the reduced PMS is easily
oxidized by oxygen, it is used in assays as an electron
carrier between enzymes and oxygen, cytochrome c,
indophenols, or tetrazolium salts. The reduced PMS is
used as an electron donor to reduce cytochrome c or
in photosynthetic experiments. PMS is reduced by
flavoproteins such as succinic dehydrogenase. PMS is
reduced non-enzymatically by NADH and NADPH.
PMS is also reduced by dithionite, sodium
borohydride, ascorbic acid, reduced ubiquinones, and
reduced vitamin K. PMS reacts with sulfhydryl groups
including those of enzymes.1
Usage of PMS for
detection of specific dehydrogenases has been
reported.2,3
Precautions and Disclaimer
For Laboratory Use Only. Not for drug, household or
other uses.
Preparation Instructions
Phenazine methosulfate is soluble in water
(approximately 200 mg/ml).
Storage/Stability
Solutions should be prepared in deionized water, and
not in neutral buffers. Solutions in water are stable
frozen and protected from light for several months.
Decomposition of solutions (with formation of
pyocyanine and other products) may take hours when
they are exposed to diffuse light, but only 5-10 minutes
when exposed to sunlight.
Solutions are stabilized by polyvalent cations. Cations
such as Ca2+, Mg2+, Mn2+, Co2+, Cu2+, Ba2+, Zn2+, and
Sn3+ at 100 mM each, prevented decomposition of
PMS solutions when stored for 1 month, but were not
protective when solutions were exposed to sunlight.1,2
Storage Temperature -0 °C
Product Description
Molecular Formula: C14H14N2O4S
Molecular Weight: 306.3
CAS Number: 299-11-6
Extinction coefficient: EM for oxidized phenazine
methosulfate (PMS) at maximum wavelength 387 nm
is 26,300 (pH 2-8). The difference in molar extinction
coefficients between oxidized and reduced PMS is
25,000.1
Melting Point: 158-160 °C
PMS is an electron acceptor and carrier in enzyme
systems. The oxidized form is yellow and the reduced
form is colorless. Since the reduced PMS is easily
oxidized by oxygen, it is used in assays as an electron
carrier between enzymes and oxygen, cytochrome c,
indophenols, or tetrazolium salts. The reduced PMS is
used as an electron donor to reduce cytochrome c or
in photosynthetic experiments. PMS is reduced by
flavoproteins such as succinic dehydrogenase. PMS is
reduced non-enzymatically by NADH and NADPH.
PMS is also reduced by dithionite, sodium
borohydride, ascorbic acid, reduced ubiquinones, and
reduced vitamin K. PMS reacts with sulfhydryl groups
including those of enzymes.1
Usage of PMS for
detection of specific dehydrogenases has been
reported.2,3
Precautions and Disclaimer
For Laboratory Use Only. Not for drug, household or
other uses.
Preparation Instructions
Phenazine methosulfate is soluble in water
(approximately 200 mg/ml).
Storage/Stability
Solutions should be prepared in deionized water, and
not in neutral buffers. Solutions in water are stable
frozen and protected from light for several months.
Decomposition of solutions (with formation of
pyocyanine and other products) may take hours when
they are exposed to diffuse light, but only 5-10 minutes
when exposed to sunlight.
Solutions are stabilized by polyvalent cations. Cations
such as Ca2+, Mg2+, Mn2+, Co2+, Cu2+, Ba2+, Zn2+, and
Sn3+ at 100 mM each, prevented decomposition of
PMS solutions when stored for 1 month, but were not
protective when solutions were exposed to sunlight.1,2
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