Biotin

Application

Biotin may be used to elute proteins from avidin/streptavidin resins.
• It has been used for culturing of oligodendrocytes.
• It has been used as a vitamin supplement for the growth of Bacillus species.
• It has been used for blocking endogenous biotin during immunohistology procedures.

Packaging

1, 5, 10, 25 g in poly bottle

100, 500 mg in poly bottle

Biochem/physiol Actions

Biotin is a small molecule and its presence with biologically active macromolecules does not affect the biological function of the macromolecule. The biotin-(strept)avidin system are required for various applications. Biotin also works as a cofactor of carboxylating enzymes, such as pyruvate carboxylase. It catalyzes the formation of oxaloacetate from pyruvate and CO₂. Biotin conjugation with avidin or streptavidin helps in linking the target molecule (antibody, nucleotide, protein A etc.) with the labeling system (enzyme, fluorescent, chemiluminescent probes). This complex is used for many detection systems, such as immunoassays, DNA hybridization assays, immunohistochemistry and flow-cytometry. This approach is also applied for the purification and characterization of various molecules of interest